Difference between revisions of "Part:BBa K4395001"
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| + | UGT-33(UDP-GlucuronosylTransferase33), located in intracellular membrane-bounded organelle, catalyzes the biosynthesis of salidroside from tyrosine. It is a member of the UGT superfamily, which is one of the largest multigene families in Arabidopsis[1]. It has a UDP-glucose-4-O-glucosyltransferase(T8GT) protein coding region. And it is the most active gene among R. rosea Tyrosol-Modifying UGTs. Four UGTs (RrUGT 17, 29, 32, and 33) were found possessing regio-specific T8GT activity for conversion of tyrosol to salidroside, amongst which RrUGT33 displayed the highest activity [2][3]. It is used as the last gene to complete salidroside biosynthesis from tyrosine. | ||
| + | Moreover, RrUGT33 exhibits the highest T8GT catalytic efficiency in four UGTs(RrUGT 2, 3, 29, and 33) with a kcat/KM value of 420.6 s−1 mM−1 and was subsequently referred to as RrT8GT[2]. | ||
| + | These characteristics of UGT-33 engaged our attention, so we chose it as one of the candidate UGTs of our experiment. | ||
| + | |||
| + | UGT33 is the most active gene among R. rosea Tyrosol-Modifying UGTs. The protein it encodes reduces tyrosol to produce salidroside. UGT33 | ||
| + | |||
| + | The UGT super-family is one of the largest enzyme families in the plant kingdom. Previous research has shown that(Michel et al., 2017) among all the genes encoding UDP-glucose-4-O-glucosyltransferase(T8GT), UGT33 is the most active T8GT. | ||
| + | References | ||
| + | [1] Li, Y. et al. (2001) ‘Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana’, JOURNAL OF BIOLOGICAL CHEMISTRY, 1 January, pp. 4338–4343. Available at: https://search.ebscohost.com/login.aspx?direct=true&db=edsbl&AN=RN091459152&site=eds-live (Accessed: 27 May 2022). | ||
| + | [2]Michael P. Torrens-Spence;Tomas Pluskal;Fu-Shuang Li;Valentina Carballo;Jing-Ke Weng (2018) ‘Complete Pathway Elucidation and Heterologous Reconstitution of Rhodiola Salidroside Biosynthesis’, 分子植物：英文版 / Molecular Plant, (1), p. 205. Available at: https://search.ebscohost.com/login.aspx?direct=true&db=edscqv&AN=edscqv.674735793&site=eds-live (Accessed: 27 May 2022). | ||
| + | [3] Obesity, Fitness & Wellness Week (2022) ‘Findings from Jiangnan University Yields New Data on Phytochemistry (Biosynthesis and Biotechnological Production of Salidroside From Rhodiola Genus Plants)’, 5 February, p. 101. Available at: https://search.ebscohost.com/login.aspx?direct=true&db=edsggo&AN=edsgcl.690958131&site=eds-live (Accessed: 27 May 2022). | ||
Latest revision as of 07:16, 4 October 2022
UGT-33(UDP-GlucuronosylTransferase33), located in intracellular membrane-bounded organelle, catalyzes the biosynthesis of salidroside from tyrosine. It is a member of the UGT superfamily, which is one of the largest multigene families in Arabidopsis[1]. It has a UDP-glucose-4-O-glucosyltransferase(T8GT) protein coding region. And it is the most active gene among R. rosea Tyrosol-Modifying UGTs. Four UGTs (RrUGT 17, 29, 32, and 33) were found possessing regio-specific T8GT activity for conversion of tyrosol to salidroside, amongst which RrUGT33 displayed the highest activity [2][3]. It is used as the last gene to complete salidroside biosynthesis from tyrosine. Moreover, RrUGT33 exhibits the highest T8GT catalytic efficiency in four UGTs(RrUGT 2, 3, 29, and 33) with a kcat/KM value of 420.6 s−1 mM−1 and was subsequently referred to as RrT8GT[2].
These characteristics of UGT-33 engaged our attention, so we chose it as one of the candidate UGTs of our experiment.
UGT33 is the most active gene among R. rosea Tyrosol-Modifying UGTs. The protein it encodes reduces tyrosol to produce salidroside. UGT33
The UGT super-family is one of the largest enzyme families in the plant kingdom. Previous research has shown that(Michel et al., 2017) among all the genes encoding UDP-glucose-4-O-glucosyltransferase(T8GT), UGT33 is the most active T8GT. References [1] Li, Y. et al. (2001) ‘Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana’, JOURNAL OF BIOLOGICAL CHEMISTRY, 1 January, pp. 4338–4343. Available at: https://search.ebscohost.com/login.aspx?direct=true&db=edsbl&AN=RN091459152&site=eds-live (Accessed: 27 May 2022). [2]Michael P. Torrens-Spence;Tomas Pluskal;Fu-Shuang Li;Valentina Carballo;Jing-Ke Weng (2018) ‘Complete Pathway Elucidation and Heterologous Reconstitution of Rhodiola Salidroside Biosynthesis’, 分子植物：英文版 / Molecular Plant, (1), p. 205. Available at: https://search.ebscohost.com/login.aspx?direct=true&db=edscqv&AN=edscqv.674735793&site=eds-live (Accessed: 27 May 2022). [3] Obesity, Fitness & Wellness Week (2022) ‘Findings from Jiangnan University Yields New Data on Phytochemistry (Biosynthesis and Biotechnological Production of Salidroside From Rhodiola Genus Plants)’, 5 February, p. 101. Available at: https://search.ebscohost.com/login.aspx?direct=true&db=edsggo&AN=edsgcl.690958131&site=eds-live (Accessed: 27 May 2022).