Difference between revisions of "Part:BBa K4463005"

 
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<partinfo>BBa_K4463005 short</partinfo>
 
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This is a improved part based on BBa_k4463001. The ability of Metallothioneins to bind metal ions depends on the amount of cysteine (Cys). Therefore, in order to improve the metal ion binding capacity of ShMT, we performed a three-point mutation of the original sequence by targeted mutagenesis to increase the Cys content.
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This is a improved part based on BBa_k4463001. The ability of Metallothioneins to bind metal ions depends on the amount of cysteine (Cys). Therefore, in order to improve the metal ion binding capacity of ShMT, we performed a three-point mutation of the original sequence by targeted mutagenesis to increase the Cys content<sup>[1]</sup>.
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<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/shmt-adsorb-cu-1800.png
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Figure1. E.coli expressed ShMT3 protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.
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<h3>Reference </h3>
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1 X. Li, Z. Ren, M. J. C. Crabbe, L. Wang and W. Ma, <i>Ecotoxicology and Environmental Safety</i>, 2021, 222, 112512.
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<!-- Add more about the biology of this part here
 
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Revision as of 06:51, 4 October 2022


ShMT3


This is a improved part based on BBa_k4463001. The ability of Metallothioneins to bind metal ions depends on the amount of cysteine (Cys). Therefore, in order to improve the metal ion binding capacity of ShMT, we performed a three-point mutation of the original sequence by targeted mutagenesis to increase the Cys content[1].

Figure1. E.coli expressed ShMT3 protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.

Reference

1 X. Li, Z. Ren, M. J. C. Crabbe, L. Wang and W. Ma, Ecotoxicology and Environmental Safety, 2021, 222, 112512.  

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]