Difference between revisions of "Part:BBa K4463002"

Revision as of 05:50, 4 October 2022

SUMO-CsgA-SmtA
SmtA is a protein of Metallothionein family, which can bind to multiple metal ions, such as Cu(II), Cd(II) and Cu(II). CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA is achieved by linking to CsgA. SUMO tag was added to improve the solubility of recombinant protein, CsgA and SmtA. Improved parts is based on part BBa_K519010.

In the IGEM project of XJTLU_2023, we added SUMO before SmtA to improve its function. We measured the amounts of binding Cu(II) and Cd(II), the results showed the function of SmtA performed better in the presence of SUMO.

Figure1. The left column is the concentration of Cu(II) adsorbed by control group that E.coli containing empty plasmid. The middle column is Cu(II) adsorbed by E.coli expressing SmtA. The right column is Cu(II) adsorbed by E.coli expressing SUMO-SmtA, which is also the part in this page. All groups grew in 1.8mM CuSO4 solution.

Figure2. The left column is the concentration of Cd(II) adsorbed by control group that E.coli containing empty plasmid. The middle column is Cd(II) adsorbed by E.coli expressing SmtA. The right column is Cd(II) adsorbed by E.coli expressing SUMO-SmtA, which is also the part in this page. All groups grew in 300μM CdCl2 solution.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 863
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 863
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 297
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 863
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 863
Illegal AgeI site found at 891
1000
COMPATIBLE WITH RFC[1000]

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-SmtA is a protein of Metallothionein family, which can bind to multiple metal ions, such as Cu(II), Cd(II) and Cu(II). CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA is achieved by linking to CsgA. SUMO tag was added to improve the solubility of recombinant protein, CsgA and SmtA. Improved parts is based on part <a href="https://parts.igem.org/Part:BBa_K519010">BBa_K519010</a>.
+SmtA is a protein of Metallothionein family, which can bind to multiple metal ions, such as Cu(II), Cd(II) and Cu(II). CsgA is the major curli subunit protein of <i>E.coli</i>. Therefor, the surface display of SmtA is achieved by linking to CsgA. SUMO tag was added to improve the solubility of recombinant protein, CsgA and SmtA. Improved parts is based on part <a href="https://parts.igem.org/Part:BBa_K519010">BBa_K519010</a>.
+<br>
+<br>
 In the IGEM project of XJTLU_2023, we added SUMO before SmtA to improve its function. We measured the amounts of binding Cu(II) and Cd(II), the results showed the function of SmtA performed better in the presence of SUMO.
+<br>
+<div>
+<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/cu-1800-adsorption.png
+" style="width:25%;height=25%; "></img>
+</div>
+Figure1. The left column is the concentration of Cu(II) adsorbed by control group that <i>E.coli</i> containing empty plasmid. The middle column is Cu(II) adsorbed by <i>E.coli</i> expressing SmtA. The right column is Cu(II) adsorbed by <i>E.coli</i> expressing SUMO-SmtA, which is also the part in this page. All groups grew in 1.8mM CuSO4 solution.
+<br>
+<div>
+<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/cd-adsorbed-results-smta.png
+" style="width:25%;height=25%; "></img>
+</div>
+Figure2. The left column is the concentration of Cd(II) adsorbed by control group that <i>E.coli</i> containing empty plasmid. The middle column is Cd(II) adsorbed by <i>E.coli</i> expressing SmtA. The right column is Cd(II) adsorbed by <i>E.coli</i> expressing SUMO-SmtA, which is also the part in this page. All groups grew in 300μM CdCl2 solution.