Difference between revisions of "Part:BBa K4298012"
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<partinfo>BBa_K4298012 short</partinfo> | <partinfo>BBa_K4298012 short</partinfo> | ||
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| + | PUC19 plasmid, containing flanked inverted repeats, and the insertion sequence is EPI with α-secretion signal. EPI from the inverted repeat can be introduced into P1 by homologous recombination at both ends. | ||
The original inverted repeat sequence at each end of the insertion fragment, which assists the integration of the insertion sequence into the P1 plasmid | The original inverted repeat sequence at each end of the insertion fragment, which assists the integration of the insertion sequence into the P1 plasmid | ||
Revision as of 05:28, 4 October 2022
pUC19-ITR-EPI
PUC19 plasmid, containing flanked inverted repeats, and the insertion sequence is EPI with α-secretion signal. EPI from the inverted repeat can be introduced into P1 by homologous recombination at both ends.
The original inverted repeat sequence at each end of the insertion fragment, which assists the integration of the insertion sequence into the P1 plasmid
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1182
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1000
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 505