Difference between revisions of "Part:BBa K4463002"

Revision as of 05:00, 4 October 2022

SUMO-CsgA-SmtA
SmtA is a protein of Metallothionein family, which can bind to multiple metal ions, such as Cu(II), Cd(II) and Cu(II). CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA is achieved by linking to CsgA. SUMO tag was added to improve the solubility of recombinant protein, CsgA and SmtA. Improved parts is based on part BBa_K519010. In the IGEM project of XJTLU_2023, we added SUMO before SmtA to improve its function. We measured the amounts of binding Cu(II) and Cd(II), the results showed the function of SmtA performed better in the presence of SUMO.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 863
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 863
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 297
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 863
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 863
Illegal AgeI site found at 891
1000
COMPATIBLE WITH RFC[1000]

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-CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA, a protein belongs to Metallothionein family, achieved by linking to csgA. SUMO tag was added to improve the solubility of recombinant protein, csgA and SmtA. Improved parts is based on part <a href="https://parts.igem.org/Part:BBa_K519010">BBa_K519010</a>.
+SmtA is a protein of Metallothionein family, which can bind to multiple metal ions, such as Cu(II), Cd(II) and Cu(II). CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA is achieved by linking to CsgA. SUMO tag was added to improve the solubility of recombinant protein, CsgA and SmtA. Improved parts is based on part <a href="https://parts.igem.org/Part:BBa_K519010">BBa_K519010</a>.
+In the IGEM project of XJTLU_2023, we added SUMO before SmtA to improve its function. We measured the amounts of binding Cu(II) and Cd(II), the results showed the function of SmtA performed better in the presence of SUMO.