Difference between revisions of "Part:BBa K4202048"
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To select and sequence the exogenous fragments introduced to plasmid pHY300PLK, we designed a pair of primers located upstream and downstream of the MCS on the plasmid backbone. This is the reverse primer downstream of the MCS. | To select and sequence the exogenous fragments introduced to plasmid pHY300PLK, we designed a pair of primers located upstream and downstream of the MCS on the plasmid backbone. This is the reverse primer downstream of the MCS. | ||
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| + | See the forward primer in <partinfo>BBa_K4202047</partinfo> | ||
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| + | <div align="center">[[File:Liu_Junyi_2-1.png|300px|]]</div> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 03:08, 4 October 2022
primer B.S - PHY - R
To select and sequence the exogenous fragments introduced to plasmid pHY300PLK, we designed a pair of primers located upstream and downstream of the MCS on the plasmid backbone. This is the reverse primer downstream of the MCS.
See the forward primer in BBa_K4202047
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]