Difference between revisions of "Part:BBa K4115009"

Revision as of 02:34, 4 October 2022

NS3-2

Neutral site 3(NS3) is commonly used in homologous recombination for S.elongatus, where NS3-1 acts as an upstream homology arm. [1]

Usage and Biology

It is used together with NS3-1 (BBa_K3228025) to construct plasmids required for S.elongatus HL7942, where NS3-2 is placed upstream of the aimed fragments.

References

[1] Niederholtmeyer H, Wolfstädter BT, Savage DF, Silver PA, Way JC. Engineering cyanobacteria to synthesize and export hydrophilic products. Appl Environ Microbiol. 2010 Jun;76(11):3462-6. doi: 10.1128/AEM.00202-10. Epub 2010 Apr 2. Erratum in: Appl Environ Microbiol. 2010 Sep;76(17):6023. PMID: 20363793; PMCID: PMC2876443.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 507
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 507
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 507
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 507
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4115009 short</partinfo>
-An upstream homology arm from <i>S.elongatus</i> .
+Neutral site 3(NS3) is commonly used in homologous recombination for <i>S.elongatus</i>, where NS3-1 acts as an upstream homology arm. [1]
 ===Usage and Biology===
-NS3-2 was used together with NS3-1 to construct plasmids required for Synechococcus.
+It is used together with NS3-1 [https://parts.igem.org/Part:BBa_K3228025 (BBa_K3228025)] to construct plasmids required for <i>S.elongatus</i> HL7942, where NS3-2 is placed upstream of the aimed fragments.
+== References ==
+<small>
+[1] Niederholtmeyer H, Wolfstädter BT, Savage DF, Silver PA, Way JC. Engineering cyanobacteria to synthesize and export hydrophilic products. Appl Environ Microbiol. 2010 Jun;76(11):3462-6. doi: 10.1128/AEM.00202-10. Epub 2010 Apr 2. Erratum in: Appl Environ Microbiol. 2010 Sep;76(17):6023. PMID: 20363793; PMCID: PMC2876443.
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