Difference between revisions of "Part:BBa K4115010"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | It is used together with NSI-DS [https://parts.igem.org/Part:BBa_K4115011 (BBa_K4115011)] to construct plasmids required for <i>S.elongatus</i> HL7942. | + | It is used together with NSI-DS [https://parts.igem.org/Part:BBa_K4115011 (BBa_K4115011)] to construct plasmids required for <i>S.elongatus</i> HL7942, where NSI-US is placed upstream of the aimed fragments. |
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Revision as of 02:09, 4 October 2022
NSI-US
An upstream homology arm from S.elongatus UTEX2973.
Usage and Biology
It is used together with NSI-DS (BBa_K4115011) to construct plasmids required for S.elongatus HL7942, where NSI-US is placed upstream of the aimed fragments.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 333
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 520
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 333
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 333
- 1000COMPATIBLE WITH RFC[1000]