Difference between revisions of "Part:BBa K4387003"
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===Results===
 
===Results===
  
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[[File:DoseResponseETH.jpeg|450px|thumb|right|'''Figure 1:''' Induction response of the 2016 ETH Team NorV promoter to different DETA/NO concentrations with respect to time.]]
 
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[[File:ResponseETH.jpeg|450px|thumb|right|'''Figure 2:''' Dose Response of the 2016 ETH Team NorV Promoter at different DETA/NO concentrations.]]
 
  
  

Revision as of 23:26, 3 October 2022


Negative Control of Nitric Oxide Sensing Genetic Circuit

Usage and Biology

This composite part consists of a superfolder GFP (BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward terminator (BBa_B0015). To assess the proper induction of our construct by nitric oxide, we removed the pNorVβ promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of the constructs BBa_K4387004, BBa_K4387005, BBa_K4387006, and BBa_K4387007.


This construct was tested in the bacterial strain E.coli Nissle 1917.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Results