Difference between revisions of "Part:BBa K4387003"
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This composite part consists of a superfolder GFP (<html><a href="http://https://parts.igem.org/Part:BBa_K2553008">BBa_K2553008</a></html>), the NorR regulator (<html><a href="http://https://parts.igem.org/Part:BBa_K4387001">BBa_K4387001</a></html>), and a double forward terminator (<html><a href="http://https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>). To assess the proper induction of our construct by nitric oxide, we removed the pNorVβ promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of the constructs <html><a href="http://https://parts.igem.org/Part:BBa_K4387004">BBa_K4387004</a></html>, <html><a href="http://https://parts.igem.org/Part:BBa_K4387005">BBa_K4387005</a></html>, <html><a href="http://https://parts.igem.org/Part:BBa_K4387006">BBa_K4387006</a></html>, and <html><a href="http://https://parts.igem.org/Part:BBa_K4387007">BBa_K4387007</a></html>. | This composite part consists of a superfolder GFP (<html><a href="http://https://parts.igem.org/Part:BBa_K2553008">BBa_K2553008</a></html>), the NorR regulator (<html><a href="http://https://parts.igem.org/Part:BBa_K4387001">BBa_K4387001</a></html>), and a double forward terminator (<html><a href="http://https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>). To assess the proper induction of our construct by nitric oxide, we removed the pNorVβ promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of the constructs <html><a href="http://https://parts.igem.org/Part:BBa_K4387004">BBa_K4387004</a></html>, <html><a href="http://https://parts.igem.org/Part:BBa_K4387005">BBa_K4387005</a></html>, <html><a href="http://https://parts.igem.org/Part:BBa_K4387006">BBa_K4387006</a></html>, and <html><a href="http://https://parts.igem.org/Part:BBa_K4387007">BBa_K4387007</a></html>. | ||
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This construct was tested in the bacterial strain E.coli Nissle 1917. | This construct was tested in the bacterial strain E.coli Nissle 1917. | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 22:48, 3 October 2022
Negative Control of Nitric Oxide Sensing Genetic Circuit
Usage and Biology
This composite part consists of a superfolder GFP (BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward terminator (BBa_B0015). To assess the proper induction of our construct by nitric oxide, we removed the pNorVβ promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of the constructs BBa_K4387004, BBa_K4387005, BBa_K4387006, and BBa_K4387007.
This construct was tested in the bacterial strain E.coli Nissle 1917.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 439
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]