Difference between revisions of "Part:BBa K4387003"
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<partinfo>BBa_K4387003 short</partinfo>
 
<partinfo>BBa_K4387003 short</partinfo>
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This composite part consists of a superfolder GFP (BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward terminator (BBa_B0015). To assess the proper induction of our construct by nitric oxide, we removed the pNorVβ promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of the constructs BBa_K4387004, BBa_K4387005, BBa_K4387006, and BBa_K4387007.
 
  
 
===Usage and Biology===
 
===Usage and Biology===
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This composite part consists of a superfolder GFP (BBa_K2553008) <html><a href="https://parts.igem.org/Part:BBa_K2553008> , the NorR regulator (BBa_K4387001), and a double forward terminator (BBa_B0015). To assess the proper induction of our construct by nitric oxide, we removed the pNorVβ promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of the constructs BBa_K4387004, BBa_K4387005, BBa_K4387006, and BBa_K4387007.
  
 
This construct was tested in the bacterial strain E.coli Nissle 1917.  
 
This construct was tested in the bacterial strain E.coli Nissle 1917.  

Revision as of 22:39, 3 October 2022


Negative Control of Nitric Oxide Sensing Genetic Circuit

Usage and Biology

This composite part consists of a superfolder GFP (BBa_K2553008)