Difference between revisions of "Part:BBa K4387003"
Line 9: Line 9:
 
This construct was tested in the bacterial strain E.coli Nissle 1917.  
 
This construct was tested in the bacterial strain E.coli Nissle 1917.  
  
 +
===Sequence and Features===
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'/span>
 
<partinfo>BBa_K4387003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4387003 SequenceAndFeatures</partinfo>
  

Revision as of 22:36, 3 October 2022


Negative Control of Nitric Oxide Sensing Genetic Circuit

This composite part consists of a superfolder GFP (BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward terminator (BBa_B0015). To assess the proper induction of our construct by nitric oxide, we removed the pNorVβ promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of the constructs BBa_K4387004, BBa_K4387005, BBa_K4387006, and BBa_K4387007.

Usage and Biology

This construct was tested in the bacterial strain E.coli Nissle 1917.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]