Difference between revisions of "Part:BBa K4387004"
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<partinfo>BBa_K4387004 short</partinfo> | <partinfo>BBa_K4387004 short</partinfo> | ||
| − | This composite part consists of the ETH promoter NorV (BBa_K2116002), superfolder GFP, the NorR regulator, and a double forward terminator. We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the | + | This composite part consists of the ETH promoter NorV (BBa_K2116002), superfolder GFP (BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward terminator (BBa_B0015). We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | This construct was tested in the bacterial strain E.coli Nissle 1917. | ||
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Revision as of 22:30, 3 October 2022
Nitric Oxide Sensing Genetic Circuit with Promoter BBa_K2116002
This composite part consists of the ETH promoter NorV (BBa_K2116002), superfolder GFP (BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward terminator (BBa_B0015). We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 742
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]