Difference between revisions of "Part:BBa K4387000"
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===Usage and Biology===
 
===Usage and Biology===
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===Design Notes===
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No codon optimization was necessary. The sequence was taken directly from the reference paper.
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===Source===
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The sequence of the pNorVβ was obtained from the paper of Xiaoyu J. Chen et al. [1]
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===References===
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[1]Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223
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Revision as of 22:11, 3 October 2022


Nitric Oxide Sensing Promoter pNorVβ

pNorVβ is an optimized nitric oxide sensitive promoter. In our constructs, this promoter is coupled to sfGFP and NorR. The corresponding integration host factor IHF2 binding site was removed from the promoter pNorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used nitric oxide source in the experiment, activating the downstream genes' transcription[1]. This promoter was tested in E.coli Nissle 1917.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]