Difference between revisions of "Part:BBa K4368002"

 
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Cex is the gene coding for the exoglucanase enzyme of Celullomonas fimi. In our case, we have incorporated this enzyme into a regulated gene construct under the control of the pcstA promoter. We have also included an export site, yebF, so that this enzyme can be expressed in the cell membrane and thus function correctly together with the enzymes of the CAZymes complex. In addition, the sequence has been optimised in order to incorporate it into the Escherichia coli organism. Finally, a double terminator has been used.
  
 
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Revision as of 19:15, 3 October 2022


bglX

Cex is the gene coding for the exoglucanase enzyme of Celullomonas fimi. In our case, we have incorporated this enzyme into a regulated gene construct under the control of the pcstA promoter. We have also included an export site, yebF, so that this enzyme can be expressed in the cell membrane and thus function correctly together with the enzymes of the CAZymes complex. In addition, the sequence has been optimised in order to incorporate it into the Escherichia coli organism. Finally, a double terminator has been used.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1297
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1207