Difference between revisions of "Part:BBa K4390010"

Revision as of 17:57, 3 October 2022

Mytilus galloprovincialis Metallothionein

Metallothionein (MT) is a small protein (around 6-7 kDa) which is rich in cysteine. These thiol group in cysteines provide ability to chelate almost all heavy metal ions including Cd2+, Hg2+, Pb2+ and As3+, but had been shown that has higher binding affinity with Hg2+ (Manceau, A. et al., 2019). The ability of chelating heavy metals provides the metal tolerance for its hosts. For its ability to binding heavy metal strongly, this part can be used to build structure which can capture heavy metal ions in aqueous environment. This MT sequence was obtained from Mytilus galloprovincialis, as an isoform which contains higher cysteine frequency with 23 in 72 amino acids (Vergani, L. et al., 2007) and designed as a C part for JUMP assembly (Valenzuela-Ortega M and French C., 2021).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4390010 short</partinfo>
-Metallothionein from Mytilus galloprovincialis. This part was designed as a JUMP C part. Metallothioneins chelate heavy metal ions, including Cd2+, Hg2+, Pb2+, As3+ and Zn2+.
+Metallothionein (MT) is a small protein (around 6-7 kDa) which is rich in cysteine. These thiol group in cysteines provide ability to chelate almost all heavy metal ions including Cd2+, Hg2+, Pb2+ and As3+, but had been shown that has higher binding affinity with Hg2+ (Manceau, A. et al., 2019). The ability of chelating heavy metals provides the metal tolerance for its hosts.
+For its ability to binding heavy metal strongly, this part can be used to build structure which can capture heavy metal ions in aqueous environment. This MT sequence was obtained from Mytilus galloprovincialis, as an isoform which contains higher cysteine frequency with 23 in 72 amino acids (Vergani, L. et al., 2007) and designed as a C part for JUMP assembly (Valenzuela-Ortega M and French C., 2021).
 <!-- Add more about the biology of this part here