Difference between revisions of "Part:BBa K4115036"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Constitutive expression of the SacC protein in E. coli is expected to confer the ability to efficiently utilize extracellular sucrose produced by cyanobacteria. | + | Constitutive expression of the SacC protein in E. coli is expected to confer the ability to efficiently utilize extracellular sucrose produced by cyanobacteria.Also involved in screening for constitutive promoters suitable for the project. |
See <partinfo>BBa_K4115017</partinfo> for further details. | See <partinfo>BBa_K4115017</partinfo> for further details. | ||
Revision as of 13:18, 3 October 2022
J23101-B0034-SacC-B0015
The J23101 promoter on this part constitutively expresses SacC protein in E. coli.
Usage and Biology
Constitutive expression of the SacC protein in E. coli is expected to confer the ability to efficiently utilize extracellular sucrose produced by cyanobacteria.Also involved in screening for constitutive promoters suitable for the project. See BBa_K4115017 for further details.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 498
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1370
Illegal PstI site found at 498 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1413
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 498
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 498
Illegal NgoMIV site found at 757
Illegal AgeI site found at 479 - 1000COMPATIBLE WITH RFC[1000]