Difference between revisions of "Part:BBa K4115039:Design"
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===Design Notes===
 
===Design Notes===
  
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The function of LuxR is first verified in E.coli, which will then be used in A.caulinodans ORS571 to build communications and regulations between E.coli and S.elongatus.
+
The function of LuxR is first verified in <i>E. coli</i>, which will then be used in A.caulinodans ORS571 to build communications and regulations between <i>E. coli</i> and <i>S.elongatus</i> HL7942.
  
 
===Source===
 
===Source===

Revision as of 12:24, 3 October 2022


J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


Design Notes

The function of LuxR is first verified in E. coli, which will then be used in A.caulinodans ORS571 to build communications and regulations between E. coli and S.elongatus HL7942.

Source

The LuxR and sfGFP fragments were synthesized by the company. The plasmid was constructed by our team.

References