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Revision as of 11:46, 3 October 2022

Peg10

This is a paternally expressed imprinted gene that binds its own mRNA and self-assembles into virion-like extracellular vesicles that encapsulate their own mRNA and are released from cells, enabling intercellular transfer of PEG10 mRNA. It is thought to have originated from the Ty3/Gypsy family of retrotransposons

Usage

PEG10 mRNA can be transferred between cells thanks to a paternally expressed imprinted gene that binds its own mRNA and self-assembles into extracellular vesicles that resemble virion capsules. Making VLPs is one of the SEND platform's components for mRNA delivery. Therefore, we took advantage of it by attaching the 5' and 3'UTR of PEG10 to the mRNA of PAH in order to make the PEG10 protein encapsulate the PAH mRNA.

Characterization of Mutational Landscape

After creating a multiple sequence alignment of the protein sequence and predicting mutational landscapes, the effect of these mutations on the evolutionary fitness of the protein is tested. The prediction of the mutational landscape by saturation mutagenesis of the Peg10 protein. The (P631D) mutation, as depicted in the chart, had the greatest score when compared to other mutations. On the other hand, it's clear that the (Q632R) had the least evolutionary fitness for Peg10 protein. As displayed in Figure(1)

Figure 1. (shows the mutational landscape of the Peg10 protein.)

References

Sequence and Features