Difference between revisions of "Part:BBa K4140013"
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==Usage== | ==Usage== | ||
| − | == | + | ==Characterization of Mutational Landscape== |
| − | + | After creating a multiple sequence alignment of the protein sequence and predicting mutational landscapes, the effect of these mutations on the evolutionary fitness of the protein is tested. The prediction of the mutational landscape by saturation mutagenesis of the VSVg-Fusogen protein. The (Y148H) mutation, as depicted in the chart, had the greatest score when compared to other mutations. On the other hand, it's clear that the (A56T) had the least evolutionary fitness for VSVg-Fusogen protein. As displayed in Figure(1) | |
| − | + | ||
| + | [[File:Vsvg.png|thumb|Right|Figure 1. (shows the mutational landscape of the VSVg-Fusogen protein.) ]] | ||
| + | <br><br><br><br><br><br><br> | ||
==References== | ==References== | ||
Revision as of 08:04, 3 October 2022
Sensing Device
The Vesicular Stomatitis Virus G (VSV G) protein is a typical type III viral fusion protein that, when present in cell-free environments, forms complexes with plasmid DNA and particles that resemble MLV retroviruses and improves DNA transfection.
Usage
Characterization of Mutational Landscape
After creating a multiple sequence alignment of the protein sequence and predicting mutational landscapes, the effect of these mutations on the evolutionary fitness of the protein is tested. The prediction of the mutational landscape by saturation mutagenesis of the VSVg-Fusogen protein. The (Y148H) mutation, as depicted in the chart, had the greatest score when compared to other mutations. On the other hand, it's clear that the (A56T) had the least evolutionary fitness for VSVg-Fusogen protein. As displayed in Figure(1)
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 771
Illegal BglII site found at 1113 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 577
- 1000COMPATIBLE WITH RFC[1000]