Difference between revisions of "Part:BBa K4115017"
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The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.
 
The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.
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[[Image:SacCgrowthcurve1.png|450px|thumb|left|'''Figure 1:''' Fermentation of ''E.&nbsp;coli'' KRXwith <partinfo>BBa_K863005</partinfo> (ECOL) in an Infors Labfors Bioreactor, scale: 3&nbsp;L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60&nbsp;µg/mL chloramphenicol, 37&nbsp;°C, pH&nbsp;7, agitation on cascade to hold pO<sub>2</sub> at 50&nbsp;%, OD<sub>600</sub> measured every 30&nbsp;minutes.]]
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[[Image:SacCgrowthcurve1.png|450px|thumb|left|'''Figure 1:''' Fermentation of ''E.&nbsp;coli'' with <partinfo>BBa_K4115017</partinfo> in 300ml conical flask in biological shaker, scale: 100&nbsp;mL M9 medium, 37&nbsp;°C, 220&nbsp;rpm, initial pH&nbsp;7.5, OD<sub>600</sub> measured every 2&nbsp;hours.]]
  
 
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Revision as of 06:57, 3 October 2022


SacC (the extracellular sucrase gene)

The sequence of Zymomonas mobilis gene sacC that encodes the extracellular sucrase. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1309
    Illegal PstI site found at 437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1352
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 437
    Illegal NgoMIV site found at 696
    Illegal AgeI site found at 418
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.

Figure 1: Fermentation of E. coli with BBa_K4115017 in 300ml conical flask in biological shaker, scale: 100 mL M9 medium, 37 °C, 220 rpm, initial pH 7.5, OD600 measured every 2 hours.