Difference between revisions of "Part:BBa K4115045"
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This composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. The core component of this composite part is cscB(BBa_K3971011), a membrane transporter that transports sucrose to the outside of the cell with the help of the H gradient. We engineered cscB into HL7942 so that it can export sucrose to be used as the carbon source for E. coli.
 
This composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. The core component of this composite part is cscB(BBa_K3971011), a membrane transporter that transports sucrose to the outside of the cell with the help of the H gradient. We engineered cscB into HL7942 so that it can export sucrose to be used as the carbon source for E. coli.
  
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[[File:Overview_of_the_construct_CMV-Cas-VP16_Freigem_2013.png|700px|]]
 
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===Usage and Biology===
 
===Usage and Biology===
  
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[[File:Overview_of_the_construct_CMV-Cas-VP16_Freigem_2013.png|700px|]]
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Revision as of 05:52, 3 October 2022


NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1

This composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. The core component of this composite part is cscB(BBa_K3971011), a membrane transporter that transports sucrose to the outside of the cell with the help of the H gradient. We engineered cscB into HL7942 so that it can export sucrose to be used as the carbon source for E. coli.

Overview of the construct CMV-Cas-VP16 Freigem 2013.png Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
    Illegal NgoMIV site found at 1678
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1722