Difference between revisions of "Part:BBa K4115045"
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===Usage and Biology===
 
===Usage and Biology===
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[[File:Overview_of_the_construct_CMV-Cas-VP16_Freigem_2013.png|700px|]]
  
 
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Revision as of 05:51, 3 October 2022


NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1

This composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. The core component of this composite part is cscB(BBa_K3971011), a membrane transporter that transports sucrose to the outside of the cell with the help of the H gradient. We engineered cscB into HL7942 so that it can export sucrose to be used as the carbon source for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
    Illegal NgoMIV site found at 1678
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1722