Difference between revisions of "Part:BBa K4387997:Design"
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===Design Notes=== | ===Design Notes=== | ||
Shortening this sequence to the essential last 60 amino acids has not been tested yet. | Shortening this sequence to the essential last 60 amino acids has not been tested yet. | ||
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Sequence of the HlyD was obtain from the paper of Ruano-Gallego et al. [1] | Sequence of the HlyD was obtain from the paper of Ruano-Gallego et al. [1] | ||
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===References=== | ===References=== | ||
[1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0 | [1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0 | ||
Revision as of 15:56, 2 October 2022
HlyA-tag obtained from the hemolysin A one-step secretion system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 565
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Shortening this sequence to the essential last 60 amino acids has not been tested yet.
Source
Sequence of the HlyD was obtain from the paper of Ruano-Gallego et al. [1]
References
[1] Ruano-Gallego, D., Fraile, S., Gutierrez, C. et al. Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Microb Cell Fact 18, 47 (2019). https://doi.org/10.1186/s12934-019-1094-0