Difference between revisions of "Part:BBa K4387005"

Revision as of 15:46, 2 October 2022

Nitric Oxide Sensing Genetic Circuit With One Ribosomal Binding Site

This composite part consists of the inducible PnorV_beta, sfGFP preceded by one ribosomal binding site, a NorR regulator, and a double forward terminator. We chose a high-copy backbone from Twist for this part. Due to the competitive binding of the activated and inactivated NorR on the promoter, we decided on this construct with a positive feedback loop that adjusted the levels of NorR. The presence of nitric oxide would activate PnorV_beta to induce GFP and NorR expression. Thereby, we ensure that high amounts of NorR will be produced in the presence of NO and in the presence of NO only.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 703
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4387005 short</partinfo>
-This composite part consists of the inducible PnorV_beta, sfGFP preceded by one ribosomal binding site, a NorR regulator, and a double forward terminator. We chose a high-copy backbone from Twist for this part. Due to the competitive binding of the activated and inactivated NorR on the promoter, we decided on this construct with a positive feedback loop that adjusted the levels of NorR. The increased amount of nitric oxide in the gut activates the PnorV_beta and starts the GFP expression. When the concentration of NorR exceeds the concentration of nitric oxide, the expression of NorR is inhibited till the NO concentration is high again.
+This composite part consists of the inducible PnorV_beta, sfGFP preceded by one ribosomal binding site, a NorR regulator, and a double forward terminator. We chose a high-copy backbone from Twist for this part. Due to the competitive binding of the activated and inactivated NorR on the promoter, we decided on this construct with a positive feedback loop that adjusted the levels of NorR. The presence of nitric oxide would activate PnorV_beta to induce GFP and NorR expression. Thereby, we ensure that high amounts of NorR will be produced in the presence of NO and in the presence of NO only.
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