Difference between revisions of "Part:BBa K4387003"

 
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<partinfo>BBa_K4387003 short</partinfo>
 
<partinfo>BBa_K4387003 short</partinfo>
  
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In order to test the exact effect of our construct, we removed the PnorV_beta promoter to create a negative control. With the removal of the promoter, no NorR should be produced; thus, the promoter will not be activated, and no GFP will be produced.  
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To assess the proper induction of our construct by nitric oxide, we removed the pNorV_beta promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of BBa_K4387005.
  
 
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Revision as of 15:29, 2 October 2022


Negative Control of Nitric Oxide Sensing Genetic Circuit

To assess the proper induction of our construct by nitric oxide, we removed the pNorV_beta promoter to create a negative control. With the removal of the promoter, the presence of nitric oxide should not affect the expression of GFP. Therefore this construct accounts for the leakiness of BBa_K4387005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]