Difference between revisions of "Part:BBa K4387000"
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PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to sfGFP and NorR. Two corresponding integration host factors IHF1 and IHF2 binding sites were removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.
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pNorVβ is an optimized nitric oxide sensitive promoter. In our constructs, this promoter is coupled to sfGFP and NorR. The corresponding integration host factor IHF2 binding site was removed from the promoter pNorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used nitric oxide source in the experiment, activating the downstream genes' transcription.
  
 
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===Usage and Biology===
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Usage and Biology
  
 
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Revision as of 15:16, 2 October 2022


Nitric Oxide Sensing Promoter pNorVβ

pNorVβ is an optimized nitric oxide sensitive promoter. In our constructs, this promoter is coupled to sfGFP and NorR. The corresponding integration host factor IHF2 binding site was removed from the promoter pNorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used nitric oxide source in the experiment, activating the downstream genes' transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]