Difference between revisions of "Part:BBa K4129002:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
The part was designed to be used with USER cloning. BsaI sites were not removed to avoid accidentally affecting the promoter activity.
+
The part was designed to be used with USER cloning. Restriction sites used in assembly standards were not removed to avoid accidentally affecting the promoter activity.
  
  

Latest revision as of 09:55, 2 October 2022


Promoter of the Aspergillus niger FDH gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 34
    Illegal SpeI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 34
    Illegal SpeI site found at 160
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 34
    Illegal SpeI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 34
    Illegal SpeI site found at 160
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 564
    Illegal BsaI site found at 929


Design Notes

The part was designed to be used with USER cloning. Restriction sites used in assembly standards were not removed to avoid accidentally affecting the promoter activity.


Source

Amplified by PCR from gDNA.

References