Difference between revisions of "Part:BBa K4180001"

Line 10: Line 10:
 
<!--??There have two different way to upload??
 
<!--??There have two different way to upload??
 
[[File:      ??put picture link in here??      ]]-->
 
[[File:      ??put picture link in here??      ]]-->
[[File:       ??put picture link in here??      |200px|thumb|left|     ??you can write the command in this part??    ]]
+
<!--[[File:BBa K4180001.png|400px|thumb|left|<h3>&emsp;&emsp;&emsp;&emsp;  BBa_K4180000 SNF1 full length <br> &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]
 +
[[File:BBa K4180000-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do SNF1 full length PCR - colonies #1, 3, 5, and 6 had BBa_K4180000(SNF1 full length)<br> &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]-->
  
  

Revision as of 08:28, 2 October 2022


pGal promoter

Gal promoter is in the plasmid and genes are cloned downstream of the pGal promoter, so the cloned genes can be induced in the presence of galactose Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]