Difference between revisions of "Part:BBa K4408006:Design"
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===Source=== | ===Source=== | ||
| − | Linker 1 is based on part | + | Linker 1 is based on part BBa_K1583012. |
hbd is based on part BBa_M1350. | hbd is based on part BBa_M1350. | ||
SpyTag is based on part BBa_K3329001 | SpyTag is based on part BBa_K3329001 | ||
===References=== | ===References=== | ||
Latest revision as of 07:36, 2 October 2022
hbd modified by oligopeptide SpyTag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Enzyme encoded by thl catalyzes acetyl-CoA into acetoacetyl-CoA which is further transformed to 3-hydroxybutytyl-CoA by hbd encoded protein in the synthesis pathway of butyl acid in Clostridium tyrobutyricum. We designed SpyCatcher-Linker 1-thl (BBa_K4408005) and SpyTag-Linker 1-hbd to link these two enzymes together through the SpyCatcher/SpyTag system. In this way, we can improve the utilization of reagents and the reaction rate of the transformation from acetyl-CoA to 3-hydroxybutytyl-CoA, enhancing the production of butyl acid.
Source
Linker 1 is based on part BBa_K1583012. hbd is based on part BBa_M1350. SpyTag is based on part BBa_K3329001