Difference between revisions of "Part:BBa K4408006"
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<partinfo>BBa_K4408006 short</partinfo> | <partinfo>BBa_K4408006 short</partinfo> | ||
| − | This part is the coding region of hbd enzyme combined with genes coding for SpyTag oligopeptide and Linker 1. It is constructed by hbd (BBa_M1350), SpyTag（BBa_3329001） and linker 1( | + | This part is the coding region of hbd enzyme combined with genes coding for SpyTag oligopeptide and Linker 1. It is constructed by hbd (BBa_M1350), SpyTag（BBa_3329001） and linker 1(BBa_K1583012) sequences. It can be bounded with SpyCatcher-Linker 1-thl (BBa_4408005) to enhance the catalysis of acetyl-CoA into 3-hydroxybutytyl-CoA by thl and hbd enzymes in the metabolic pathway of Clostridium tyrobutyricum. |
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Latest revision as of 06:48, 2 October 2022
hbd modified by oligopeptide SpyTag
This part is the coding region of hbd enzyme combined with genes coding for SpyTag oligopeptide and Linker 1. It is constructed by hbd (BBa_M1350), SpyTag（BBa_3329001） and linker 1(BBa_K1583012) sequences. It can be bounded with SpyCatcher-Linker 1-thl (BBa_4408005) to enhance the catalysis of acetyl-CoA into 3-hydroxybutytyl-CoA by thl and hbd enzymes in the metabolic pathway of Clostridium tyrobutyricum.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]