Difference between revisions of "Part:BBa K4408005"
 
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<partinfo>BBa_K4408005 short</partinfo>
 
<partinfo>BBa_K4408005 short</partinfo>
  
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This part is the coding region of thl enzyme combined with genes coding for SpyCatcher oligopeptide and Linker 1. It is constructed by thl (BBa_K3443003), SpyCatcher (BBa_K3329016) and Linker 1(BBa_K4408003) sequences. It can be bounded with SpyTag-Linker 1-hbd (BBa_K4408006) to enhance the catalysis of acetyl-CoA into 3-hydroxybutytyl-CoA by thl and hbd enzymes in the metabolic pathway of Clostridium tyrobutyricum.
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This part is the coding region of thl enzyme combined with genes coding for SpyCatcher oligopeptide and Linker 1. It is constructed by thl (BBa_K3443003), SpyCatcher (BBa_K3329016) and Linker 1(BBa_K1583012) sequences. It can be bounded with SpyTag-Linker 1-hbd (BBa_K4408006) to enhance the catalysis of acetyl-CoA into 3-hydroxybutytyl-CoA by thl and hbd enzymes in the metabolic pathway of Clostridium tyrobutyricum.
  
 
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Latest revision as of 06:47, 2 October 2022


thl modified by oligopeptide SpyCatcher

This part is the coding region of thl enzyme combined with genes coding for SpyCatcher oligopeptide and Linker 1. It is constructed by thl (BBa_K3443003), SpyCatcher (BBa_K3329016) and Linker 1(BBa_K1583012) sequences. It can be bounded with SpyTag-Linker 1-hbd (BBa_K4408006) to enhance the catalysis of acetyl-CoA into 3-hydroxybutytyl-CoA by thl and hbd enzymes in the metabolic pathway of Clostridium tyrobutyricum.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1076
    Illegal SapI.rc site found at 900