Difference between revisions of "Part:BBa K4380011:Design"

 
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===Design Notes===
 
===Design Notes===
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The design of this part used exploitet type IIs restriction enzymes LguI ability to recognize asymmetric DNA sequences and cleave at a defined distance outside of itsrecognition sequence.
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The design of this part used exploited type IIs restriction enzymes LguI ability to recognize asymmetric DNA sequences and cleave at a defined distance outside of its recognition sequence.
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+
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===Source===
 
===Source===

Revision as of 21:07, 1 October 2022


eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

The design of this part used exploited type IIs restriction enzymes LguI ability to recognize asymmetric DNA sequences and cleave at a defined distance outside of its recognition sequence.

Source

Synthetic

References