Difference between revisions of "Part:BBa K4432010"

 
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<partinfo>BBa_K4432010 short</partinfo>
 
<partinfo>BBa_K4432010 short</partinfo>
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This part is a synthetic RBS specifically designed for the ''Nostoc punctiforme'' CrtW ([[Part:BBa_K4432020|BBa_K4432020]]) using the [https://www.denovodna.com/ Salis Lab RBS Library Calculator v2.0] [1-3].
  
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===Usage and Biology===
 
===Usage and Biology===
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This RBS was used to drive the expression of CrtW ([[Part:BBa_K4432020|BBa_K4432020]]) under the control of the T5 [[Part:BBa_K4432000|BBa_K4432000]] or pLac (Part:BBa_R0010|BBa_R0010) promoters in the composite parts [[Part:BBa_K4432120|BBa_K4432120]], [[Part:BBa_K4432220|BBa_K4432220]] and [[Part:BBa_K4432320|BBa_K4432320]].
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Using the Salis Lab RBS Library Calculator v2.0 [1-3], the predicted features of this RBS are:
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Translation Initiation Rate (au) : 183001.2231
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dG_total (kcal/mol) : -11.10937396
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dG_mRNA_rRNA (kcal/mol) : -63.4313505
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dG_spacing (kcal/mol) : 0.005325582
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dG_stacking (kcal/mol) : 0
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dG_standby (kcal/mol) : 0.138149285
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dG_start (kcal/mol) : -2.76
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dG_mRNA (kcal/mol) : -55.24000168
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Accuracy (warnings) : none
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This RBS was selected as predicted to be the strongest from a library of 108 RBSes (HAGHAAKGVTAAGTAAGGWGGTATAA) for which the estimated Translation Initiation Rates (TIR) range from 292.31 to 183001.22
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===References===
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[1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.
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[2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.
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[3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.
  
 
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Revision as of 21:04, 1 October 2022


Synthetic RBS designed for CrtW This part is a synthetic RBS specifically designed for the Nostoc punctiforme CrtW (BBa_K4432020) using the Salis Lab RBS Library Calculator v2.0 [1-3].

Usage and Biology

This RBS was used to drive the expression of CrtW (BBa_K4432020) under the control of the T5 BBa_K4432000 or pLac (Part:BBa_R0010|BBa_R0010) promoters in the composite parts BBa_K4432120, BBa_K4432220 and BBa_K4432320.

Using the Salis Lab RBS Library Calculator v2.0 [1-3], the predicted features of this RBS are: 

Translation Initiation Rate (au)	:	183001.2231
dG_total (kcal/mol)	:	-11.10937396
dG_mRNA_rRNA (kcal/mol)	:	-63.4313505
dG_spacing (kcal/mol)	:	0.005325582
dG_stacking (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0.138149285
dG_start (kcal/mol)	:	-2.76
dG_mRNA (kcal/mol)	:	-55.24000168
Accuracy (warnings)	:	none

This RBS was selected as predicted to be the strongest from a library of 108 RBSes (HAGHAAKGVTAAGTAAGGWGGTATAA) for which the estimated Translation Initiation Rates (TIR) range from 292.31 to 183001.22


References

[1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.

[2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.

[3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]