Difference between revisions of "Part:BBa K4115046"

Revision as of 14:55, 1 October 2022

NS3-2-KanR-J23101-B0034-LuxR-B0015-Lux pR-B0034-sfGFP-B0015-NS3-1

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This Composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules, and sfGFP is designed here to detect the response intensity of LuxR. The higher the response intensity, the more sfGFP is expressed, and the more obvious the fluorescence is. Thus, we can construct a communicating system between E.coli and Synechococcus.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 507
Illegal PstI site found at 4261
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1167
Illegal NheI site found at 1190
Illegal PstI site found at 507
Illegal PstI site found at 4261
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 507
Illegal PstI site found at 4261
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 507
Illegal PstI site found at 4261
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2145

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+This Composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules, and sfGFP is designed here to detect the response intensity of LuxR. The higher the response intensity, the more sfGFP is expressed, and the more obvious the fluorescence is. Thus, we can construct a communicating system between E.coli and Synechococcus.
 <!-- Add more about the biology of this part here