Difference between revisions of "Part:BBa K4385011:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of | + | This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of copper ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein. |
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===Source=== | ===Source=== |
Revision as of 13:27, 1 October 2022
Lead sensor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 462
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 462
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 462
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 462
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 253
Illegal BsaI.rc site found at 1394
Design Notes
This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of copper ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein.
Source
It is amplified from E. coli genomic DNA.