Difference between revisions of "Part:BBa K4169015:Design"

 
m
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Codon optimization.
+
Codon optimization，add His tag.
  
  

Revision as of 12:12, 1 October 2022


produce TMADH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 388
    Illegal XhoI site found at 1717
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 388
    Illegal PstI site found at 183
    Illegal PstI site found at 1782
    Illegal AgeI site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization，add His tag.


Source

It comes from https://www.ebi.ac.uk/ena/browser/view/CDX46666 Sequence: X89575.1.

References