Difference between revisions of "Part:BBa K4408007"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4408007 short</partinfo> | ||
+ | |||
+ | This part is the coding region of the fusion protein of Candida antarctica: lipase B (CALB) and chitin-binding domain (ChBD). It consists CALB (BBa_K2302006), Linker 2 (BBa_K4408004) and ChBD BBa_T2028) sequences. Candida antarctica lipase B (CALB) is a widely used lipase with high stereoselectivity and stability. ChBD is an affinity tag for chitin purification of proteins. ChBD-fusion CALB can facilitate the purification of CALB, thus enhancing the reaction rate of CALB catalyzed esterification. | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4408007 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K4408007 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 11:50, 1 October 2022
Lipase CALB connected with chitin binding domain
This part is the coding region of the fusion protein of Candida antarctica: lipase B (CALB) and chitin-binding domain (ChBD). It consists CALB (BBa_K2302006), Linker 2 (BBa_K4408004) and ChBD BBa_T2028) sequences. Candida antarctica lipase B (CALB) is a widely used lipase with high stereoselectivity and stability. ChBD is an affinity tag for chitin purification of proteins. ChBD-fusion CALB can facilitate the purification of CALB, thus enhancing the reaction rate of CALB catalyzed esterification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 949
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 670
Illegal NgoMIV site found at 780
Illegal AgeI site found at 472
Illegal AgeI site found at 730 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1025
Illegal SapI site found at 964
Illegal SapI site found at 994