Difference between revisions of "Part:BBa K4220100"

(→‎Introduction)
(→‎Others)
Line 21: Line 21:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
  
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4220100 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4220100 SequenceAndFeatures</partinfo>
−
 
  
 
==References==
 
==References==

Revision as of 07:02, 1 October 2022


HSF1up-HIS3+NatR+ADH1+CUP1-HSF ORF

Introduction

This is a kill switch in yeast which can let the yeast survive only in the presence of copper ion.

How to kill the yeast?

HSF1: essential gene in yeast [1]

How to determine when to kill?

Copper ion: abundant in the intestine; scarce in the environment [2]

Others

HIS3-NatR-ADH1: resistant gene expression for screening

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 247
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 367
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 512

References

1 Kopczynski, J. B., Raff, A. C. & Bonner, J. J. Translational readthrough at nonsense mutations in the HSF1 gene of Saccharomyces cerevisiae. Mol Gen Genet 234, 369-378, doi:10.1007/BF00538696 (1992).

2 Nose, Y., Kim, B. E. & Thiele, D. J. Ctr1 drives intestinal copper absorption and is essential for growth, iron metabolism, and neonatal cardiac function. Cell metabolism 4, 235-244, doi:10.1016/j.cmet.2006.08.009 (2006).