Difference between revisions of "Part:BBa K200005:Design"

(→‎Design Notes)
(→‎Design Notes)
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Backward: <b>GGACTAGTA</b>CTACGCAAGCTTTGGAAAG      <br>
 
Backward: <b>GGACTAGTA</b>CTACGCAAGCTTTGGAAAG      <br>
 
Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang)
 
Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang)
−
<br>
+
<br><br>
 
<i> BioBrick overhang shown in bold </i>
 
<i> BioBrick overhang shown in bold </i>
  

Revision as of 13:25, 2 September 2009

OtsA: Part 1 of 2 for trehalose producing enzymes.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 383
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR from Escherichia Coli BL21(DE3) using Pfu Ultra II Polymerase.


Forward: GCTCTAGATGAGTCGTTTAGTCGTAG
Recomended Temperatures for PCR : 52.4C(without overhang) and 63.2(with overhang)

Backward: GGACTAGTACTACGCAAGCTTTGGAAAG
Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang)

BioBrick overhang shown in bold

Source

Escherichia coli BL21(DE3)

References