Difference between revisions of "Part:BBa K200005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | PCR from Escherichia Coli BL21(DE3) | + | PCR from Escherichia Coli BL21(DE3) using Pfu Ultra II Polymerase. |
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Backward: <b>GGACTAGTA</b>CTACGCAAGCTTTGGAAAG <br> | Backward: <b>GGACTAGTA</b>CTACGCAAGCTTTGGAAAG <br> | ||
Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang) | Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang) | ||
| + | <br> | ||
| + | <i> BioBrick overhang shown in bold </i> | ||
===Source=== | ===Source=== | ||
Revision as of 13:25, 2 September 2009
OtsA: Part 1 of 2 for trehalose producing enzymes.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 383
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR from Escherichia Coli BL21(DE3) using Pfu Ultra II Polymerase.
Forward: GCTCTAGATGAGTCGTTTAGTCGTAG
Recomended Temperatures for PCR : 52.4C(without overhang) and 63.2(with overhang)
Backward: GGACTAGTACTACGCAAGCTTTGGAAAG
Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang)
BioBrick overhang shown in bold
Source
Escherichia coli BL21(DE3)