Difference between revisions of "Part:BBa K200008"
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=References=
 
=References=
 
[http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta Sequence from NCBI]
 
[http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta Sequence from NCBI]
−
<biblio>#PAH1 pmid=2014036 </biblio>
+
 
−
<biblio>#PAH2 pmid=15708798 </biblio>
+
<biblio>
−
<biblio>#PAH3 pmid=8573072</biblio>
+
#PAH1 pmid=2014036
−
<biblio>#PAH4 pmid=13525410 </biblio>
+
#PAH2 pmid=15708798
 +
#PAH3 pmid=8573072
 +
#PAH4 pmid=13525410
 +
</biblio>

Revision as of 12:30, 2 September 2009

Phenylalanine hydroxylase

[http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase Phenylalanine hydroxylase (PAH)] is the enzyme that breaks down [http://en.wikipedia.org/wiki/Phenylalanine phenylalanine] to [http://en.wikipedia.org/wiki/Tyrosine tyrosine]. Deficiency of this enzyme activity results in the autosomal recessive disorder [http://en.wikipedia.org/wiki/Phenylketonuria phenylketonuria].#PAH1

PAH works well at a PH of around 7.0 (similar to the small intestine), and is denatured at temperatures above 30 degrees celsius (in experiments with gram-negative bacteria)#PAH2. The enzyme can be rendered protease resistant via full phosphorylation.#PAH3

Furthermore, the presence of oxygen and an additional cofactor (TPNH) is required for the hydrolysis to take place #PAH4.

Limitation: Note that this part only works on gram-negative bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

[http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta Sequence from NCBI]

<biblio>

  1. PAH1 pmid=2014036
  2. PAH2 pmid=15708798
  3. PAH3 pmid=8573072
  4. PAH4 pmid=13525410

</biblio>