Difference between revisions of "Part:BBa K200007"

Revision as of 11:23, 2 September 2009

Cellulase

This is a BioBrick for cellulase production in E.coli. This biobrick codes for endo-b-1,4-glucanase E (CelE) cellulase, which is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.1

Cellulase mainly catalyses the reactions that changes crystalline cellulose to cellobiose2 and then finally to glucose. It also catalyses, to a small extent, the break down of carboxymethyl cellulose. This cellulase is protease resistant.

As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise cellulase to a tunable threshold. Following that, E.coli is to be self-encapsulated so as to protect cellulase through the stomach, until its release in the small intestine. Cellulase will then be able to digest the cellulose found in our food.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 199
Illegal PstI site found at 700
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 700
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 287
Illegal BamHI site found at 814
Illegal XhoI site found at 524
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 199
Illegal PstI site found at 700
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 199
Illegal PstI site found at 700
1000
COMPATIBLE WITH RFC[1000]

References

[http://www.ncbi.nlm.nih.gov/nuccore/125972525?report=genbank&log$=seqview&from=961597&to=964041 Sequence from NCBI] <biblio>

1 pmid=9055408
2 pmid=10714996

</biblio>

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K200007 short</partinfo>
-This is a BioBrick for cellulase production in E.coli. This biobrick codes for CelE cellulase, which is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.<cite>1</cite>
+This is a BioBrick for cellulase production in E.coli. This biobrick codes for endo-b-1,4-glucanase E (CelE) cellulase, which is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.<cite>1</cite>
@@ Line 16: / Line 16: @@
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+It can be used in gram positive bacteria.
+The enzyme has a C-end cellulosome-binding domain (CBD), which is used to deliver its resident catalytic domain
+to the cellulosome. The cellulosome is multi-subunit complex that is involved in the hydrolysis of crystalline cellulose. [http://www.springerlink.com/content/lp8g657n13g76187/fulltext.pdf 3]
 <!-- -->