Difference between revisions of "Part:BBa K4207057:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K4207056 short</partinfo>
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<partinfo>BBa_K4207057 short</partinfo>
  
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<partinfo>BBa_K4207056 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4207057 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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This toehold switch was designed according to the B-series toehold structure (Pardeee et. al., 2016). The A-series structure was designed to reduce translational leakage compared to older toehold switches (Green et. al., 2014). The binding site of the toehold switch was designed to bind to a conserved sequence in the BYDV genome, allowing it to sense the presence of this trigger sequence. This toehold has a downstream 21-nt linker sequence, which ends in the codon AAG. To prevent frame-shifting, the last G is omitted from the documented sequence and the junction site should have the sequence GATG instead of the standard AATG junction site
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This toehold switch was designed according to the A-series toehold structure (Pardeee et. al., 2016). The A-series structure was designed to reduce translational leakage compared to older toehold switches (Green et. al., 2014). The binding site of the toehold switch was designed to bind to a conserved sequence in the BYDV genome, allowing it to sense the presence of this trigger sequence. This toehold has a downstream 21-nt linker sequence, which ends in the codon AAG. To prevent frame-shifting, the last G is omitted from the documented sequence and the junction site should have the sequence GATG instead of the standard AATG junction site.
  
  
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===Source===
 
===Source===
  
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This part was created by us, screening SPLCV whole genomes for conserved sequences to be detected by the toehold switches.
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This part was created by us, screening TMV whole genomes for conserved sequences to be detected by the toehold switches.
  
 
===References===
 
===References===

Revision as of 19:38, 30 September 2022


TMV toehold switch A1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 24


Design Notes

This toehold switch was designed according to the A-series toehold structure (Pardeee et. al., 2016). The A-series structure was designed to reduce translational leakage compared to older toehold switches (Green et. al., 2014). The binding site of the toehold switch was designed to bind to a conserved sequence in the BYDV genome, allowing it to sense the presence of this trigger sequence. This toehold has a downstream 21-nt linker sequence, which ends in the codon AAG. To prevent frame-shifting, the last G is omitted from the documented sequence and the junction site should have the sequence GATG instead of the standard AATG junction site.


Source

This part was created by us, screening TMV whole genomes for conserved sequences to be detected by the toehold switches.

References