Difference between revisions of "Part:BBa K200006:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 9: Line 9:
  
 
Primers Used:<br>
 
Primers Used:<br>
−
Forward:  
+
Forward: <br>
−
<b>GCTCTAG</b>ATGACAGAACCGTTAACCG
+
<b>GCTCTAG</b>ATGACAGAACCGTTAACCG <br>
 
Recomended Temperatures for PCR : 54.5C(without overhang) and 64.8C(with overhang)
 
Recomended Temperatures for PCR : 54.5C(without overhang) and 64.8C(with overhang)
−
 
+
<br><br>
−
Reverse:  
+
Reverse:<br>
−
<b>GGACTAGTA</b>TTAGATACTACGACTAAACGAC
+
<b>GGACTAGTA</b>TTAGATACTACGACTAAACGAC<br>
−
Recomended Temperatures for PCR : 54.7C(without overhang) and 64.2C(with overhang)
+
Recomended Temperatures for PCR : 54.7C(without overhang) and 64.2C(with overhang)<br>
 +
<i>Biobrick Overhang shown in Bold</i>
  
 
===Source===
 
===Source===

Revision as of 13:12, 1 September 2009

OtsB: Part 2 of 2 coding for trehalose producing enzymes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR from Escherichia Coli BL21(DE3)

Primers Used:
Forward:
GCTCTAGATGACAGAACCGTTAACCG
Recomended Temperatures for PCR : 54.5C(without overhang) and 64.8C(with overhang)

Reverse:
GGACTAGTATTAGATACTACGACTAAACGAC
Recomended Temperatures for PCR : 54.7C(without overhang) and 64.2C(with overhang)
Biobrick Overhang shown in Bold

Source

Coding sequence isolated from e-coli

References