Difference between revisions of "Part:BBa K200006:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K200006 short</partinfo>
 
<partinfo>BBa_K200006 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Used to resist dessication in cells
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PCR from Escherichia Coli BL21(DE3)
  
 +
Primers Used:<br>
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Forward:
 +
<b>GCTCTAG</b>ATGACAGAACCGTTAACCG
 +
Recomended Temperatures for PCR : 54.5C(without overhang) and 64.8C(with overhang)
  
 +
Reverse:
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<b>GGACTAGTA</b>TTAGATACTACGACTAAACGAC
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Recomended Temperatures for PCR : 54.7C(without overhang) and 64.2C(with overhang)
  
 
===Source===
 
===Source===

Revision as of 13:11, 1 September 2009

OtsB: Part 2 of 2 coding for trehalose producing enzymes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR from Escherichia Coli BL21(DE3)

Primers Used:
Forward: GCTCTAGATGACAGAACCGTTAACCG Recomended Temperatures for PCR : 54.5C(without overhang) and 64.8C(with overhang)

Reverse: GGACTAGTATTAGATACTACGACTAAACGAC Recomended Temperatures for PCR : 54.7C(without overhang) and 64.2C(with overhang)

Source

Coding sequence isolated from e-coli

References