Difference between revisions of "Part:BBa K200009"
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<partinfo>BBa_K200009 short</partinfo>
 
<partinfo>BBa_K200009 short</partinfo>
  
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Dam Sensitive Restriction Enzyme.
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DpnII is a dam sensitive restriction enzyme.<br>
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The recognition sequence of this restriction enzyme is GATC, and this enzyme is a 4 base cutting enzyme leaving a 4 base overhang.
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The recognition sequence of this restriction enzyme is GATC and is a 4 base cutter, leaving a 4 base overhang.
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<br>
 
<br>
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The Cutting Site is as shown:
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The cutting site is as shown:
 
<br>
 
<br>
 
NGATCN  ->  N / GATCN <br>
 
NGATCN  ->  N / GATCN <br>

Revision as of 12:30, 1 September 2009

DpnII Composite

DpnII is a dam sensitive restriction enzyme.
The recognition sequence of this restriction enzyme is GATC and is a 4 base cutter, leaving a 4 base overhang.


The cutting site is as shown:
NGATCN -> N / GATCN
NCTAGN -> NCTAG / N


As part of the Imperial 2009 iGEM E.ncapsulator project, DpnII was used to cut up the genetic material within the cell, completing the cell death module.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]