Difference between revisions of "Part:BBa K200003"

(Usage and Biology)
(Usage and Biology)
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K200003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K200003 SequenceAndFeatures</partinfo>
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<br><br>
  
 
===Requirements===
 
===Requirements===

Revision as of 10:16, 1 September 2009

Rfal
Waal ligase is an enzyme responsible for the ligation of an O-antigen to the core oligosaccharide in the Gram-negative bacterium's outer membrane.

Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the core oligosaccharide, in K-12 it links colanic acid to the core oligosaccharide. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation.

Usage and Biology

Figure 1: LPS structure (source: [http://en.wikipedia.org/wiki/O_antigen wikipedia])

LipoPolySaccharyde is a major component of the outer cell wall in Gram-negative bacteria. It is constituted of three main components: the O-antigen, the core polysaccharides and the lipid A (see Fig. 1).
The O-antigen is a highly variable chain of polysaccharydes and is physically linked to the core.
The core component is made up of a short chain of sugars (oligosaccharide) and is linked to both the O-antigen and the Lipid A components of the cell wall.
The Lipid A component of the LPS contains fatty acid chains which causes the LPS to dock into the outer membrane of the Gram-negative bacterium.







Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 754
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Requirements

  • Gram-negative bacterium
  • Colanic acid producing gene (rcsB)


References

<biblio>

  1. function pmid=15838026
  2. Function2 pmid=19019161

</biblio>