Difference between revisions of "Part:BBa K200003"
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Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the core oligosaccharide, in K-12 it links colanic acid to the core oligosaccharide. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation. | Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the core oligosaccharide, in K-12 it links colanic acid to the core oligosaccharide. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
[[Image:II09_LPS.jpg | Thumb | left | <i>Figure 1:</i> LPS structure (source: [http://en.wikipedia.org/wiki/O_antigen wikipedia]]] | [[Image:II09_LPS.jpg | Thumb | left | <i>Figure 1:</i> LPS structure (source: [http://en.wikipedia.org/wiki/O_antigen wikipedia]]] | ||
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The core component is made up of a short chain of sugars (oligosaccharide) and is linked to both the O-antigen and the Lipid A components of the cell wall. | The core component is made up of a short chain of sugars (oligosaccharide) and is linked to both the O-antigen and the Lipid A components of the cell wall. | ||
The Lipid A component of the LPS contains fatty acid chains which causes the LPS to dock into the outer membrane of the Gram-negative bacterium. | The Lipid A component of the LPS contains fatty acid chains which causes the LPS to dock into the outer membrane of the Gram-negative bacterium. | ||
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<span class='h3bb'><b>Sequence and Features</b></span> | <span class='h3bb'><b>Sequence and Features</b></span> | ||
<partinfo>BBa_K200003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K200003 SequenceAndFeatures</partinfo> | ||
Revision as of 09:46, 1 September 2009
Rfal
Waal ligase is an enzyme responsible for the ligation of an O-antigen to the core oligosaccharide in the Gram-negative bacterium's outer membrane.
Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the core oligosaccharide, in K-12 it links colanic acid to the core oligosaccharide. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation.
Usage and Biology
![Figure 1: LPS structure (source: [http://en.wikipedia.org/wiki/O_antigen wikipedia]](/File:II09_LPS.jpg)
LipoPolySaccharyde is a major component of the outer cell wall in Gram-negative bacteria. It is constituted of three main components: the O-antigen, the core polysaccharides and the lipid A (see Fig. 1). The O-antigen is a highly variable chain of polysaccharydes and is physically linked to the core. The core component is made up of a short chain of sugars (oligosaccharide) and is linked to both the O-antigen and the Lipid A components of the cell wall. The Lipid A component of the LPS contains fatty acid chains which causes the LPS to dock into the outer membrane of the Gram-negative bacterium.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 754
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Requirements
- Gram-negative bacterium
- Colanic acid producing gene (rcsB)
References
<biblio>
- function pmid=15838026
- Function2 pmid=19019161
</biblio>