Difference between revisions of "Part:BBa K4159011:Design"
VeeraKurki (Talk | contribs) (→Design Notes) |
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For ordering the part, some changes might be needed in the beginning of the sequence as the beginning is very TA heavy. A few GC nucleotides will help the production to succeed when you order the part as a whole. | For ordering the part, some changes might be needed in the beginning of the sequence as the beginning is very TA heavy. A few GC nucleotides will help the production to succeed when you order the part as a whole. | ||
| − | + | Description: This part consists of two promoters pBAD, which is a continuous promoter, and of P2, which is an inducible promoter. We have placed the two promoters in two different direction, P2 being reversed and pBAD in the left to right direction. This way we try to avoid false positive results with the downstream genes, incase either promoter is a little "leaky". Between the promoter we have a spacer sequence and after both promoters we have placed the B0034 RBS sequence. | |
===Source=== | ===Source=== | ||
Revision as of 18:26, 30 September 2022
pBAD and reversed P2 promoters
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 239
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 179
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For ordering the part, some changes might be needed in the beginning of the sequence as the beginning is very TA heavy. A few GC nucleotides will help the production to succeed when you order the part as a whole.
Description: This part consists of two promoters pBAD, which is a continuous promoter, and of P2, which is an inducible promoter. We have placed the two promoters in two different direction, P2 being reversed and pBAD in the left to right direction. This way we try to avoid false positive results with the downstream genes, incase either promoter is a little "leaky". Between the promoter we have a spacer sequence and after both promoters we have placed the B0034 RBS sequence.
Source
The parts have been taken from the iGEM part registry.